Efficiency analysis of TP antibody detection by CLIAkits made (in China) domestically
OBJECTIVE
To investigate the scientific efficiency of TP antibody detection by CLIA kits and consider whether or not the CLIA kits made in China is appropriate for scientific use.
METHODS
1200 samples have been collected from Beijing Hospital together with 300 samples with confirmed TP an infection and 900 wholesome management samples. To detect the TP antibody of the 1200 sanples individually by the CLIA kits and the ELISA kits on the similar time. The check outcomes have been analyzed with statistical strategies.
RESULTS
The sensitivity and specificity of the CLIA kits have been 99.3% and 99.9% respectively, and constructive predictive worth of 99.7%, adverse predictive worth of 100%. With the ELISA methodology, the constructive coincidence fee was 98.7%, the adverse coincidence fee was 99.8%, and the overall coincidence fee was 99.5%.
CONCLUSIONS
The CLIA kits confirmed good scientific efficiency and the settlement fee with the ELISA kits was. The CLIA kits are appropriate for scientific use.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Gelsolin (GS) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Gelsolin (GS) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Gelsolin (GS) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Gelsolin (GS) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Human Gelsolin (GS)Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Gelsolin (GS) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Gelsolin (GS) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Gelsolin (GS) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Gelsolin (GS) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Gelsolin (GS) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: A sandwich ELISA kit for detection of Gelsolin from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: A sandwich ELISA kit for detection of Gelsolin from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Gelsolin (GS) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Gelsolin (GS) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Gelsolin (GS) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Gelsolin (GS) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Cattle Gelsolin (GS) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Gelsolin (GS) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Gelsolin (GS) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Gelsolin (GS) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Gelsolin (GS) in serum, plasma and other biological fluids.
Serum HER-2 dedication utilizing a centauer HER-2/neu equipment (CLIA methodology) in metastatic breast most cancers
Using CLIA and EIA strategies concurrently, we decided serum HER-2/neu ranges a complete of 92 occasions in 51 sufferers with metastatic breast most cancers(MBC)and three sufferers with non-recurrent breast most cancers, and in contrast the degrees measured by each strategies with the extent of IHC-staining for HER-2 and the scientific course.
Amongst 20 sufferers with IHC HER-2/3+ MBC (together with FISH+MBC), 14(70%)confirmed excessive ranges by the CLIA methodology >>cut-off worth of 15.2 ng/mL), whereas solely 4(20%)revealed excessive ranges by the EIA methodology>>cut-off worth of 6.5 ng/mL). Not one of the sufferers with CR or non-recurrent breast most cancers exhibited excessive ranges by both methodology. Some IHC HER-2(-) sufferers additionally regularly confirmed excessive ranges by the CLIA methodology.
The EIA methodology not solely revealed low-level sensitivity, but additionally was topic to interference(abnormally low ranges)as a result of trastuzumab administration. The outcomes obtained by the CLIA methodology have been in settlement with the scientific course. In 93 MBC sufferers(besides CR sufferers)whose serum HER-2 ranges have been decided by the CLIA methodology, the preliminary HER-2 ranges have been in contrast with the CEA and CA15-Three ranges.
Of the 32 IHC HER-2/3+ sufferers, 25, 13, and 12 have been famous to have excessive serum ranges of HER-2, CEA, and CA15-3, respectively. These outcomes point out that the serum HER-2 degree as assessed by the CLIA methodology is probably the most delicate marker of HER-2-positive MBC.
Growth of anti-Müllerian hormone immunoassay primarily based on biolayer interferometry know-how.
Anti-Müllerian hormone (AMH) is a biomarker for the evaluation of feminine fertility. The correct measurement of the focus of AMH is related for the success of assisted reproductive therapies and prognosis of scientific instances.
On this examine, we present that cytokines akin to fetal liver tyrosine kinase Three ligand (Flt3L), CC subtype chemokine ligand 20 (CCL20), granulocyte-macrophage colony-stimulating issue (GM-CSF), and β2-microglobulin (β2M) considerably improve the immune response in opposition to AMH.
Two anti-AMH monoclonal antibodies (mAbs) with excessive affinity have been chosen by biolayer interferometry (BLI) know-how for utility in a completely automated magnetic chemiluminescence immunoassay (CLIA). This strong and fast assay can effectively detect AMH within the vary of 0.125~20 ng mL-1 with a detection restrict of 0.099 ng mL-1.
This immunoassay confirmed excessive specificity with no cross-reaction with structurally associated proteins and a few of the different members of the TGF-β tremendous household, akin to inhibin A, activin A, follicle-stimulating hormone, and luteinizing hormone. The typical restoration charges of three completely different batches have been 100.19%, 102.72%, and 103.59%, respectively, with coefficients of variation of lower than 12%.
The developed assay was utilized within the detection of AMH in 69 serum samples from randomly chosen sufferers. Our knowledge confirmed a excessive correlation with these obtained utilizing commercially obtainable ELISA kits (correlation coefficient, 0.9831). Therefore, we advise that this immunoassay might discover utility within the growth of POCT for the prognosis of AMH in scientific samples. Graphical summary.
Description: A competitive ELISA for quantitative measurement of Human hemoglobin (Hb) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse hemoglobin (Hb) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat hemoglobin (Hb) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine hemoglobin (Hb) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey hemoglobin (Hb) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine hemoglobin (Hb) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit hemoglobin (Hb) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat hemoglobin (Hb) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig hemoglobin (Hb) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human hemoglobin (Hb) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human hemoglobin (Hb) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Human Hemoglobin (HB) in serum, plasma, tissue homogenates, erythrocyte lysates, cell culture supernates and other biological fluids.
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Human Hemoglobin (HB) in serum, plasma, tissue homogenates, erythrocyte lysates, cell culture supernates and other biological fluids.
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Human Hemoglobin (HB) in serum, plasma, tissue homogenates, erythrocyte lysates, cell culture supernates and other biological fluids.
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Human Hemoglobin (HB) in serum, plasma, tissue homogenates, erythrocyte lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Human Hemoglobin (HB) in samples from serum, plasma, tissue homogenates, erythrocyte lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: Quantitative competitive ELISA kit for measuring Human Hemoglobin (Hb) in samples from serum, plasma, lysateforRBC. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitative competitive ELISA kit for measuring Human Hemoglobin (Hb) in samples from serum, plasma, lysateforRBC. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A competitive Inhibition ELISA kit for detection of Hemoglobin from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Hemoglobin (Hb) in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: A competitive ELISA for quantitative measurement of Mouse hemoglobin (Hb) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse hemoglobin (Hb) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat hemoglobin (Hb) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat hemoglobin (Hb) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Goat Hemoglobin (HB) in serum, plasma, erythrocyte lysates.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Goat Hemoglobin (HB) in serum, plasma, erythrocyte lysates.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Goat Hemoglobin (HB) in serum, plasma, erythrocyte lysates.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Goat Hemoglobin (HB) in serum, plasma, erythrocyte lysates.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Goat Hemoglobin (HB) in samples from Serum, plasma, erythrocyte lysates. with no significant corss-reactivity with analogues from other species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Horse Hemoglobin (HB) in serum, plasma, erythrocyte lysates.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Horse Hemoglobin (HB) in serum, plasma, erythrocyte lysates.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Horse Hemoglobin (HB) in serum, plasma, erythrocyte lysates.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Horse Hemoglobin (HB) in serum, plasma, erythrocyte lysates.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Horse Hemoglobin (HB) in samples from Serum, plasma, erythrocyte lysates. with no significant corss-reactivity with analogues from other species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Hemoglobin (HB) in serum, plasma and erythrocyte lysates.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Hemoglobin (HB) in serum, plasma and erythrocyte lysates.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Hemoglobin (HB) in serum, plasma and erythrocyte lysates.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Hemoglobin (HB) in serum, plasma and erythrocyte lysates.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Hemoglobin (HB) in samples from serum, plasma and erythrocyte lysates with no significant corss-reactivity with analogues from other species.
Description: Quantitative competitive ELISA kit for measuring Mouse hemoglobin (Hb) in samples from serum, plasma, LysateforRBC. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitative competitive ELISA kit for measuring Mouse hemoglobin (Hb) in samples from serum, plasma, LysateforRBC. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitative competitive ELISA kit for measuring Pig hemoglobin (Hb) in samples from serum, plasma, lysateforRBC. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitative competitive ELISA kit for measuring Pig hemoglobin (Hb) in samples from serum, plasma, lysateforRBC. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A competitive ELISA for quantitative measurement of Canine hemoglobin (Hb) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine hemoglobin (Hb) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey hemoglobin (Hb) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey hemoglobin (Hb) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine hemoglobin (Hb) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine hemoglobin (Hb) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit hemoglobin (Hb) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
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