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Comparison of Procalcitonin Assays on KRYPTOR and LIAISON

tronolab
October 1, 2020 Didier Trono 0 Comments

Speedy and delicate detection of interleukin-6 in serum through time-resolved lateral movement immunoassay.

Interleukin 6 (IL-6) is an interleukin that acts as each a proinflammatory and anti inflammatory cytokine. It may be used as a possible diagnostic biomarker for sepsis. The intention of this research was to determine an easy-to-use detection equipment for speedy, quantitative and on-site detection of IL-6.

To develop the brand new IL-6 quantitative detecting equipment, a double-antibody sandwich immunofluorescent assay was employed primarily based on europium nanoparticles (Eu-np) mixed with lateral movement immunoassay (LFIA). The efficiency of the brand new developed equipment was evaluated within the points of parallel evaluation, linearity, sensitivity, precision, accuracy, specificity and scientific pattern evaluation.

Two-hundred and fourteen serum samples had been used to hold out the scientific pattern evaluation. The brand new IL-6 quantitative detecting equipment exhibited a large linear vary (2-500 pg/mL) and a superb sensitivity (0.37 pg/mL). The intra-assay coefficient of variation (CV) and the inter-assay CV had been 5.92%-8.87% and seven.59%-9.04%, respectively. The restoration charges ranged from 102% to 106%.

Moreover, a excessive correlation (n = 214, r = 0.9756, p < 0.01) was obtained compared with SIEMENS CLIA IL-6 equipment. Thus, the brand new quantitative technique for detecting IL-6 has been efficiently established. The outcomes indicated that the newly-developed strip primarily based on Eu-np mixed with LFIA was a facile, quick, extremely delicate, low-cost, dependable biosensor and appropriate for speedy and point-of-care take a look at (POCT) for IL-6 in serum.

 

tronolab
tronolab
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A Mannequin Primarily based on the Mixture of IFN-γ, IP-10, Ferritin and 25-Hydroxyvitamin D for Discriminating Latent From Energetic Tuberculosis in Kids.

In recent times, pediatric analysis on tuberculosis (TB) has centered on addressing new biomarkers with the potential for use as immunological non-sputum-based strategies for the analysis of TB in youngsters.

The intention of this research was to characterize a set of cytokines and a collection of particular person elements (ferritin, 25-hydroxyvitamin D [25(OH)D], parasite infections, and dietary standing) to evaluate totally different patterns for discriminating between lively TB and latent TB an infection (LTBI) in youngsters. The degrees of 13 cytokines in QuantiFERON-TB Gold In-Tube (QFT-GIT) supernatants had been analyzed in 166 youngsters: 74 with lively TB, 37 with LTBI, and 55 uninfected controls.

All cytokines had been quantified utilizing Luminex or ELISA. Ferritin and 25(OH)D had been additionally evaluated utilizing CLIA, and Toxocara canis Ig-G antibodies had been detected with a business ELISA equipment. The mixture of IP-10, IFN-γ, ferritin, and 25(OH)D achieved one of the best diagnostic efficiency to discriminate between lively TB and LTBI circumstances in youngsters in relation to the realm beneath receiver working attribute (ROC) curve 0.955 (confidence interval 95%: 0.91-1.00), attaining optimum sensitivity and specificity for the event of a brand new take a look at (93.2 and 90.0%, respectively).

Kids with TB confirmed greater ferritin ranges and an inverse correlation between 25(OH)D and IFN-γ ranges. The mannequin proposed features a mixture of biomarkers for discriminating between lively TB and LTBI in youngsters to enhance the accuracy of TB analysis in youngsters. This mix of biomarkers may need potential for figuring out the onset of main TB in youngsters.

Comparability of Procalcitonin Assays on KRYPTOR and LIAISON® XL Analyzers.

Our laboratory performs procalcitonin (PCT) assays on a Brahms KRYPTOR analyzer with the Brahms PCT delicate Kryptor equipment. On this research, we needed to match the assays obtained on this means with those carried out on the LIAISON® XL.

From January to Might 2017, 171 samples had been analyzed, of which 65 from feminine sufferers (age: 22-98 years) and 106 from male sufferers (age: 16-97 years). The PCT willpower was carried out utilizing the LIAISON® XL and KRYPTOR analyzers, by chemiluminescence (Chemiluminescence immunoassay-CLIA) (LIAISON® BRAHMS PCT® II GEN) and immunofluorescence (Brahms PCT delicate Kryptor) assay, respectively. For the LIAISON® BRAHMS PCT® II GEN, 52% of the outcomes had been positioned between 0.Zero and 0.5 ng/mL, 18% between 0.5 and a couple of.Zero ng/mL, and 30% between 2.Zero and 100 ng/mL; the imply was 4.09 ng/mL, the median 0.456 ng/mL, the utmost worth 97.2 ng/mL, and the minimal worth 0.02 ng/mL.

For the Brahms PCT delicate Kryptor, 55% of the outcomes had been positioned between 0.Zero and 0.5 ng/mL, 21% between 0.5 and a couple of.Zero ng/mL, and 24% between 2.Zero and 100 ng/mL; the imply was 3.72 ng/mL, the median 0.39 ng/mL, the utmost worth 103 ng/mL, and the minimal worth 0.01 ng/mL. The imply of the outcomes obtained with the 2 strategies confirmed no important variations (3.717 for Kryptor and 4.094 for LIAISON®). PCT assay with Brahms reagents, each on the Kryptor and LIAISON®XL platforms, presents glorious efficiency by way of sensitivity and specificity.

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GP615 GenDepot 25 plates
EUR 212

COOLSINK XT 96U, 96 WELL U-BOTTOM PLATE MODULE

432071 CORNING 1/pk
EUR 345
Description: BioCision; BioCision CoolBox

Block for 96 deep-well plate, WSC-2620/30

4002661 Atto 1unit
EUR 382
Description: Bl ock for WSC-2620 / 2630

SINGLE BLOCK, 96 WELL PCR PLATE, SKIRTED OR NONSKIRTED

480130 CORNING 1/pk
EUR 223
Description: Lab Equipment; Constant Temperature Equipment

DUAL BLOCK, 96 WELL MICROTITER PLATE OR 4 SLIDES

480131 CORNING 1/pk
EUR 218
Description: Lab Equipment; Constant Temperature Equipment

DUAL BLOCK, 96 WELL PCR PLATE, SKIRTED OR NONSKIRTED

480132 CORNING 1/pk
EUR 218
Description: Lab Equipment; Constant Temperature Equipment

Goat anti-Mouse, Coated White 96 Well Plate, 1EA

X010-1EA Arbor Assays 1EA
EUR 109

Goat anti-Rabbit, Coated White 96 Well Plate, 1EA

X014-1EA Arbor Assays 1EA
EUR 109

Goat anti-Rabbit, Coated Clear 96 Well Plate, 1EA

X016-1EA Arbor Assays 1EA
EUR 109

2 x Black Half Area 96 Well Plate, 2EA

X037-2EA Arbor Assays 2EA
EUR 94

Donkey anti-Sheep, Coated Clear 96 Well Plate, 1EA

X061-1EA Arbor Assays 1EA
EUR 109

Donkey anti-Sheep, Coated White 96 Well Plate, 1EA

X063-1EA Arbor Assays 1EA
EUR 109

96 WELL BARCODED H1-H12, CLEAR PCR PLATE FOR ABI, 0,2ML

PCR-96-AB-C-PBC CORNING 10/pk
EUR 195
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

Estrone (E1) CLIA Kit

20-abx190015 Abbexa
  • EUR 7645.00
  • EUR 4074.00
  • EUR 942.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Shipped within 5-7 working days.

DH10B Chemically Competent Cells (ig10B) - 1x96 well plate - 20µl/well

1018-96 Intact Genomics 1/EA
EUR 518

96-well Plasmid ezFilter Mini Kit

K1325-96-1 Biovision
EUR 370

96-well Plasmid ezFilter Mini Kit

K1325-96-4 Biovision
EUR 968

Goat anti-Mouse, 1 x Whole Well, Clear Coated 96 Well Plate, 1EA

X011-1EA Arbor Assays 1EA
EUR 102

Clear 384 Well Plate

X105-1EA Arbor Assays 1EA
EUR 112

Human Estrone ELISA Kit, 96 tests, Quantitative

1925 Alpha Diagnostics 1 kit
EUR 590

96 Well Human Adult Normal Genomic DNA Plate - Peripheral Blood Leukocyte

D8234148-1 Biochain 1 plate
EUR 256

Mouse anti-Cystatin C Clear Coated 96 Well Plate, 1EA

C033-1EA Arbor Assays 1EA
EUR 178

Mouse anti-Endothelin-1 Clear Coated 96 Well Plate, 1EA

C161-1EA Arbor Assays 1EA
EUR 178

DNA-BIND™ PLATE, 96 WELL, CLEAR, FLAT 1/10

2525 CORNING 1/pk
EUR 137
Description: Genomics; Genomic Plates

96 Well 0.2ml PCR Plate, Chimney-Top 10/Bag, Sterile

PCR-960-P-CH-S BBI Biotech 1PK, 10UNIT
EUR 92.79
  • Product category: Labware/Multiwell Plates/96-Well

Protein A-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack

PRTA55-5P Alpha Diagnostics 1 PK
EUR 263

Protein G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack

PRTG65-5P Alpha Diagnostics 1 PK
EUR 263

96 WELL CLEAR PCR MICROPLATE

PCR-96-C CORNING 10/pk
EUR 145
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

Goat anti-Mouse, 1 x 8 Strip Well, Clear Coated 96 Well Plate, 1EA

X012-1EA Arbor Assays 1EA
EUR 109

96-Well CometSlides

4253-096-03 Biotechne 1 Tests
EUR 142.4

Estrone-3-Sulfate (E1S) EIA Kit (One Plate)

K038-H1 Arbor Assays 1x96 well plate
EUR 533

Estrone-3-Sulfate (E1S) EIA Kit (Five Plate)

K038-H5 Arbor Assays 5x96 well plate
EUR 1980

Estrone-3-Glucuronide (E1G) EIA Kit (One Plate)

K036-H1 Arbor Assays 1x96 well plate
EUR 327

Estrone-3-Glucuronide (E1G) EIA Kit (Five Plate)

K036-H5 Arbor Assays 5x96 well plate
EUR 1088

General Estrone (E1)CLIA Kit

CCB003Ge-10x96wellstestplate Cloud-Clone 10x96-wells test plate
EUR 5490.72
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of General Estrone (E1) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Competitive Chemiluminescent immunoassay for detection of General Estrone (E1) in serum, plasma and other biological fluids.

General Estrone (E1)CLIA Kit

CCB003Ge-1x48wellstestplate Cloud-Clone 1x48-wells test plate
EUR 539.82
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of General Estrone (E1) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Competitive Chemiluminescent immunoassay for detection of General Estrone (E1) in serum, plasma and other biological fluids.

General Estrone (E1)CLIA Kit

CCB003Ge-1x96wellstestplate Cloud-Clone 1x96-wells test plate
EUR 728.32
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of General Estrone (E1) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Competitive Chemiluminescent immunoassay for detection of General Estrone (E1) in serum, plasma and other biological fluids.

General Estrone (E1)CLIA Kit

CCB003Ge-5x96wellstestplate Cloud-Clone 5x96-wells test plate
EUR 2977.44
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of General Estrone (E1) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Competitive Chemiluminescent immunoassay for detection of General Estrone (E1) in serum, plasma and other biological fluids.

General Estrone (E1) CLIA Kit

4-CCB003Ge Cloud-Clone
  • EUR 5541.00
  • EUR 2978.00
  • EUR 729.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
  • Known also as Estrone Clia kit. Alternative names of the recognized antigen: Oestrone
Description: Competitive Inhibition chemiluminescent immunoassay for detection of General Estrone (E1)Serum, plasma and other biological fluids

Estrone Sulfate (E1S) CLIA Kit

20-abx490039 Abbexa
  • EUR 8835.00
  • EUR 4701.00
  • EUR 1083.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Shipped within 20 working days.

Human Estrone ELISA kit

E01E0517-96 B-Gene 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Human Estrone in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

PCR 96-Plate unskirted

A28239 GeneOn 25 pcs
EUR 98

96 WELL BARCODED H1-H12, CLEAR PCR FULL SKIRT PCR AMPLIFICATION MICRO PLATE

PCR-96-FS-C-PBC CORNING 10/pk
EUR 168
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

Protein A&G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack

PRTAG75-5P Alpha Diagnostics 1 PK
EUR 286

96-Well 2ml Deep Plate, Natural, Edge Filled, 10 Plates/Bag

BR581-96N BBI Biotech 1PK, 10UNIT
EUR 94.85
  • Product category: Labware/Multiwell Plates/96-Well

Random Nanofibers 24 Well Plate

3D00006-24 Neuromics 700 nm-PCLs
EUR 101

Random Nanofibers 348 Well Plate

3D00006-384 Neuromics 700 nm-PCLs
EUR 104

Random Nanofibers 6 Well Plate

3D00006-6 Neuromics 700 nm-PCLs
EUR 93

Aligned Nanofibers 24 Well Plate

3D00012-24 Neuromics 700 nm-PCLs
EUR 104

Aligned Nanofibers 348 Well Plate

3D00012-384 Neuromics 700 nm-PCLs
EUR 123

Aligned Nanofibers 6 Well Plate

3D00012-6 Neuromics 700 nm-PCLs
EUR 97

Adapter for 24 well plate

3510146 Atto 1unit
EUR 5581

384 Well Plate, 10/Bag

PCR-384-SN BBI Biotech 1PK, 10UNIT
EUR 93.5
  • Product category: Labware/Multiwell Plates/384-Well

Tissue Culture Plate, 12 Well

TCP20-12 BBI Biotech 1 UNIT
EUR 53.48
  • Product category: Labware/Culture Related/Culture Plates - Multiwell

Tissue Culture Plate, 48 Well

TCP20-48 BBI Biotech 1 UNIT
EUR 53.48
  • Product category: Labware/Culture Related/Culture Plates - Multiwell

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January 2021
M T W T F S S
 123
45678910
11121314151617
18192021222324
25262728293031
« Oct    

Categories

  • Assay Kits
  • Clia Kits
  • Culture Cells
  • DNA
  • Enzymes
  • My Blog
  • NATtrol
  • PCR

Recent Posts

  • Growth of anti-Müllerian hormone immunoassay primarily based on biolayer interferometry
  • Chemiluminescence Immunoassay for the Detection of Antibodies
  • Comparison of Procalcitonin Assays on KRYPTOR and LIAISON
  • Validation of a chemiluminescent assay for specific SARS-CoV-2 antibody
  • Growth of a novel chemiluminescence immunoassay for the detection of procalcitonin

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